Devi, K. D. and Punyarani, K. and Singh, N. S. and Devi, H. S. (2013) An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications. SpringerPlus, 2. p. 669.

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Abstract

Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from different plant species of same order and DNA of the associated viruses. Here, we describe a rapid, efficient and universal protocol for isolating total DNA from the members of Zingiberales which harbor a high amount of polysaccharides and secondary metabolites. DNA isolated with this protocol was successfully used for PCR based downstream applications viz. random amplified polymorphic DNA (RAPD), Inter-simple sequence repeats (ISSR), DNA barcoding gene (Internal transcribed spacer and trnl-f) amplification and detection of the viruses.

Item Type:	Article
Subjects:	Plant Bioresources
Depositing User:	Unnamed user with email alok@urdip.res.in
Date Deposited:	09 Apr 2015 04:48
Last Modified:	09 Apr 2015 04:48
URI:	http://ibsd.sciencecentral.in/id/eprint/7

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